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M9470101.TXT
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1994-07-02
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Document 0101
DOCN M9470101
TI Detection of anti-human immunodeficiency virus type 1 (HIV-1)
immunoglobulin G in urine by an ultrasensitive enzyme immunoassay
(immune complex transfer enzyme immunoassay) with recombinant reverse
transcriptase as an antigen.
DT 9409
AU Hashinaka K; Hashida S; Hirota K; Saitoh A; Nakata A; Shinagawa H; Oka
S; Shimada K; Ishikawa E; Department of Biochemistry, Medical College of
Miyazaki, Japan.
SO J Clin Microbiol. 1994 Mar;32(3):819-22. Unique Identifier : AIDSLINE
MED/94253354
AB Anti-human immunodeficiency virus type 1 immunoglobulin G in urine was
detected by an immunoassay with reverse transcriptase as the antigen and
beta-D-galactosidase as the label; this immunoassay was 30-fold more
sensitive than the previous immunoassay with peroxidase as the label.
The sensitivity and specificity were both 100%. The lowest signal for
asymptomatic carriers was 20-fold higher than the highest signal for
seronegative subjects.
DE beta-Galactosidase Adolescence Adult Aged Antigens Evaluation
Studies Human HIV Antibodies/*URINE HIV
Seropositivity/DIAGNOSIS/IMMUNOLOGY HIV-1/*IMMUNOLOGY IgG/URINE
*Immunoenzyme Techniques/STATISTICS & NUMER DATA Middle Age
Recombinant Proteins/IMMUNOLOGY Reverse Transcriptase/IMMUNOLOGY
Sensitivity and Specificity JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).